MAB-9

WormBase ID: WBGene00003106

WormBase description: Is an ortholog of human TBX20 (T-box transcription factor 20). Is predicted to have DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II activating transcription factor binding activity; and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including cell fate specification; nematode male tail tip morphogenesis; and post-embryonic hindgut morphogenesis. Localizes to nucleus. Is expressed in several structures, including B.a; B.p; neurons; proctodeum; and rectal epithelial cell. Human ortholog(s) of this gene are implicated in several diseases, including X-linked cleft palate with or without ankyloglossia; adrenocorticotropic hormone deficiency; and dysostosis (multiple).

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TGATAGAGATGATAAAGATT

sgRNA2: CAAGTGTTTTCTGGCAGAAA

5'HR: GCATGCATTAATCTTCAGAAAAGTTAAGCATTTCTGAAATTTGACGAAATTTCTAAAAATTGAAAGAATTTTCAAAATTTCAAAATTGGTCATTATTTAATTTTTTAAAATTGAAACAATAAAATATTTTGTTCGAATTCTATTAATAGTCATTAATTTAATATATTTAATATACCACGTAACTTTTCGAAAAATTCAGGTCAAAATTTGACTGGAAATATAAATTTCCATCAAAATTACGAGGTTTTGAAATTTTTTCCAAACTTTAATTTTTTTTCGAAATTTTGGAATATTCGAAAAAACTAAGGTTTTCAATAATTTTTTATTTGTTAAAACTGTGTTTTAATTACATTTCATGTAAGTTTGAAATTAAAAAAAAATTTCAGCCTTGAAATTTCAATTGAAAAATCCAGAAAAATAGCCTCACAAAATTCAATAAATTTGGTGTAAAAACGTAAGTTTTGATGATTTTTATTTTGATTTTTTTGTAAAAAAACATAACATTGCTTTAAAGTCAAAAATAAACAGGAAATATTGATTTTCAGCAAAGTTTAAAAATTCTAGATTATTTTTAACAACTCCTAAAATTTTCAAAATTATAAATTCATTTATTTTTAAAACAATCTTAAATTTAAGAATCTAAAGTATTCAATTAAAATTATGGAACATCTGGACCCCAGTTTTCCCTTCAGAAGCTCCTCCCACCTCCCTTTCTGCAATTGGCCAGCTCCGCCCATTTACTGGCGGAGGGGTGCTTGTGAAATCGGAATTCACACTTATATATTCTCAAGCTATTCTCTAACGTATTCTTCTTTTAATTCGGGGAAAATATCTGAAAATTGTCAGAAAAATAACTGGAAATTTTTATAGTCCCGGAGCTTCTTATTCCATAAGATACTAAATTTTGAAAAAAAGGTACC

3'HR: CTGCAGATGTCAAAAAGATGTGCAAGTGATAGAGATGATAAGGATTCAGAGCCCGTTAAGGTAAGCTTGGCTGAAAGTTTTTTAAAAGGATTTGTGGAGTTTTGAACTATTTGACTGAAATATTAAACAAGTGTTTTCTGGCAGAAAACGTGTATTTTCAGCGTTAAAACTGCTAAAACCCGGTTTGAAGCTATCTAAAAAGACAAGAAAATCAATAATAAAGTTATCTGGAAAGATTTGTAATTTCTCGTATTTTAAGGGAATTTCTCTTACCATTTTTTGTAATAAAACACCTCAAAACTCCCAAAACGCCTCCAAAACCCAAATTTTCATTCCAGAAACCCCGTTTTTCGATTGCCAACATTCTTGATGAAGTAGAAGACGAGGAAGATGTGGAAGTTGATGTTGAAGACGTGGATGACGTGGATTTATCATCAATTCCATCGAAAAGTCCTGAAAGATCAAGGGGTCGGCCGAAGATTGGCTTGAAAATGAAGGTGGGCTACTCAGTTCATTTTTTATTTGTACTGAAATTAATAGAAAATATAAGTTTCGATAAATTTGCTTCTTGAACCAACTTTCTTGGATTCAAATGAACAAAGTTATATGAAACAACAGCGTGGCTTTAATATTGGCTGAGTAGCGGCTCAATGACTCCGTATCACACTAATTCATACACAAATCGACGCAAAATTTTAGGATCTCAAACTTAGTCTTTTTATCAAAACTCTAGCGTCAAAATGAGCACATATATGGCGATTTGAAATATTTGGCTGAGTAGCGACCTTTTTTTGCTGAAAAGCCCAAAATTTTCAATAAAATTTGGCAAGATTTGAGGAAACTCCGTATCACCTGGGTCGCTACTCAGCAAATTATCAAAATTGTTTTAGCTTTGTTCATTTTGGTCATAAAGAATTGAAACAAAATACATTGTGTAGCTCATAAAAATTTGCGTCGATTCGTGTTAAATTCGAATTTTATACGGAGTCCTTGAGTCGCTACTCAGCCAATATTGACAAAAAACGAGAGTTAGCCCACAATTTTCAAATTTTTTGTCTAAAAACACTAGT

KI verification primers: MAB-9-NKIF: 5'-TTTTCGCTTGAATTTAGCGAGA-3' MAB-9-NKIR: 5'-TCGATTGGTAGATTTCCTTCCTG-3'

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Strain name: SYS610

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; mab-9 (devKi182 [mNeonGreen::mab-9]) II

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-6, COG-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2, NFYA-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NFYA-1, LSY-2