Table S1. Genes analyzed in this study

Table S2. Quantitative cellular behaviors during normal embryogenesis

Table S3. Quantitative cellular behaviors of KD embryos

Table S4. Cell defects detected in KD embryos

Table S5. Overlap between gene expression status and cellular defects induced by gene KD

Table S6. Cellular defects detected in additional KD and mutant embryos

Table S7. Evaluation of the correction or alleviation of various cellular defects

Table S8. CND-1::GFP expression level

Table S9. Influence of cell modules on position defects

Control phenotypic measurement

Control RNAi raw date

Raw RNAi data

Raw RNAi data add

Lineage file
Folder	Embryos contained
Raw_data_Control	Total 105 embryos of control RNAi
Raw_data_RNAi	Total 2075 embryos of 698 RNAi genes
Raw_data_RNAi_add	Total 146 embryos of control and 15 RNAi genes

Raw image
Folder	Genotype contained
RW10434_control	Control RNAi
RW10434_RNAi_1	abhd-11.2, abts-1, acdh-1, acdh-3, acin-1, acl-11, acox-1 (acox-1.1), acs-13, adsl-1, afd-1, aha-1, ahcy-1, aho-3, akir-1, apb-3
RW10434_RNAi_2	apg-1, aph-1, aph-2, aps-3, arch-1, ari-1 (ari-1.1), asd-2, aspm-1, atg-4.1, atg-5, atm-1, B0025.4, B0041.5, B0205.6 (nfs-1), B0207.6
RW10434_RNAi_3	B0261.1, B0511.12, B0511.13 (mppe-1), B0511.7, bli-4, blmp-1, bub-1, C01A2.4, C06A5.3, C09D4.4, C10H11.8 (mlst-8), C15C6.4 (popl-4), C16C2.4, C17E4.6, C18E3.5 (snrp-40.2)
RW10434_RNAi_4	C25A1.1, C25A1.4 (sup-46), C25A1.5 (fath-1), C27A12.2 (szy-5), C27A12.6 (ari-1.3), C27A12.7 (ari-1.2), C27A12.9 (pigo-1), C30F12.1 (rege-1), C30F12.2, C30H7.2 (erp-44.1), C32E8.5 (pmlr-1), C32E8.9, C34B2.10 (spcs-1), C34B2.6 (lonp-1), C34B2.8
RW10434_RNAi_5	C34B7.2 (figo-1), C37A2.6, C37A2.7, C41D11.3, C43E11.9, C44E4.5, C45G3.3 (lipt-1), C47B2.2, C48B6.2, C50F2.3 (syf-1), C53D5.1, C53D5.5, C53H9.2, calm-1, car-1
RW10434_RNAi_6	cars-1, cas-2, ccb-1, cco-1 (cox-5B), ccpp-1, cdc-25.1, cdc-48.3, cdc-5L, cdc-7, cdk-8, cdk-9, cdt-1, cebp-2, ced-1, ced-12
RW10434_RNAi_7	ceh-45, ceh-6, cel-1, ces-1, ces-2, cey-2, cey-3, cfim-1, chaf-2, chd-1, chd-7, chn-1, chp-1, cir-1, cogc-3
RW10434_RNAi_8	cogc-5, coq-1, cpb-3, cpd-1, cpz-1, crml-1, crn-1, csnk-1, ctg-1, ctn-1, cup-2, cyc-1, cye-1, D1007.15, D1007.5 (tmem-39)
RW10434_RNAi_9	D1037.1, D1081.9 (zhp-2), D2005.3, D2030.2, D2030.4, dad-1, daf-16, daf-25, daf-8, dapk-1, dbr-1, dcp-66, dhc-1, dhfr-1, dhhc-3
RW10434_RNAi_10	dhod-1, dhp-1, dhs-3, dhs-4, did-2, dip-2, dkf-1, dnc-6, dnj-21, dnj-28, dnj-30, dnj-4, dod-18, dog-1, dot-1.1
RW10434_RNAi_11	dpf-3, drh-3, drsh-1, dut-1, dxbp-1, dyb-1, dylt-1, E01A2.5 (dph-6), E03H4.8, eaf-1, ears-1, eat-16, egl-30, eif-3.C, eif-3.E
RW10434_RNAi_12	eif-3.H, eif-6, ekl-4, elpc-1, ent-6, ercc-1, eri-7, erm-1, ero-1, eva-1, exo-3, exoc-7, exoc-8, exos-3, F02E9.7
RW10434_RNAi_13	F10G8.8, F13G3.6, F13G3.7, F15D3.6, F16A11.1, F22D6.2, F22G12.4, F23C8.5, F25H2.12, F25H2.4, F25H2.6, F26A3.1, F26A3.7, F26B1.2 (hrpk-1), F26E4.7
RW10434_RNAi_14	F26H9.2, F26H9.5, F27D4.1, F27D4.4, F27D4.7, F28B3.5 (mfsd-13.1), F30A10.3, F30A10.9, F31C3.3, F31C3.4 (zipt-1), F32A7.4 (metl-17), F33D11.10, F33H2.2, F33H2.3, F33H2.6
RW10434_RNAi_15	F36A2.2, F36A2.9, F37F2.2, F39B2.3, F39H11.1, F39H2.3, F43G9.12, F43G9.3 (slc-25A42), F45H11.5 (cox-19), F46F11.1, F52A8.5 (gcsh-2), F52A8.6 (kbrl-1), F52B5.3, F53F10.1, F53F10.2
RW10434_RNAi_16	F54D7.2 (ept-1), F55A3.3 (spt-16), F55F8.2, F57B10.5 (tmed-3), F57B10.9 (spg-20), F59A3.4 (zipt-11), F59C6.12, F59C6.5, fasn-1, fbp-1, fce-1, fcp-1, fdps-1, fhod-1, fkb-5
RW10434_RNAi_17	fkb-7, fkb-8, frm-1, frm-4, ftn-2, gcy-28, gei-17, gfi-2, gld-1, gld-2, glrx-10, gna-2, goa-1, gon-2, gpb-2
RW10434_RNAi_18	gpc-2, gpi-1, grh-1, gsk-3, gst-43, guk-1, haf-6, hasp-1, hcp-6, herc-1, hex-4, hgo-1, him-1, hinf-1, hint-1
RW10434_RNAi_19	hlh-16, hlh-2, hmbx-1, hmg-20, hmp-2, hop-1, hpo-11, hpo-13, hpo-27, hpo-3 (gpaa-1), hprt-1, hrdl-1, hrp-2 (hrpr-1), hrpf-1, hsf-1
RW10434_RNAi_20	hsp-12.1, hsr-9, hum-1, hus-1, iars-2, idha-1, iftb-1 (eif-2beta), ile-1, imb-1, imp-1, impt-1, inst-1 (ints-1), irx-1, jmjc-1, jph-1
RW10434_RNAi_21	K02B12.5 (tnsl-1), K02B12.7, K04F10.7, K07A1.3, K07A1.9 (ate-1), K07A12.1, K07A12.4 (hbs-1), K09H9.2 (dscc-1), K10C3.4, K11B4.2 (blos-9), K11D2.5 (mter-4), K12C11.1, kal-1, kin-10, kin-32
RW10434_RNAi_22	klp-15, klp-16, kpc-1, ksr-2, larp-5, lars-2, lbp-5, lbp-6, lem-3, let-355, let-381, let-49, let-504, let-526, let-607
RW10434_RNAi_23	let-611, lid-1, lin-28, lin-35, lin-41, lin-53, lin-61, lmd-1, lmn-1, lpd-2, lpd-3, lpd-5, lpd-6, lrk-1, lsm-6
RW10434_RNAi_24	M01A10.1 (fubl-4), M01E11.1, M01E11.2 (ctnb-1), M01E5.2, M04F3.4, M04F3.5, M05B5.4, maco-1, mafr-1, mag-1, magu-3, mans-1, marc-3, mat-1, mau-2
RW10434_RNAi_25	mbtr-1, mce-1, mcm-4, mcrs-1, mdt-18, mec-8, mex-3, mfap-1, mfb-1, mig-1, mppa-1, mrpl-13, mrpl-15, mrpl-17, mrpl-19
RW10434_RNAi_26	mrpl-24, mrpl-34, mrpl-47, msh-2, msh-6, mtcu-1, mtm-1, mtx-1, mus-101, mus-81, nab-1, ncbp-2, ndk-1, nduf-6, nduf-7
RW10434_RNAi_27	ndx-4, ndx-7, ned-8, ngp-1, nhr-49, nhr-69, nhx-8, nkb-1, nmat-2, nmur-4 (trhr-1), npp-12, npp-14, npp-2, nra-2, nrd-1
RW10434_RNAi_28	nubp-1, nuo-2, ola-1, otub-2, pab-1, pabp-2, pad-1, pamn-1, par-6, pars-2, pash-1, pbrm-1, pbs-2, pcbd-1, pck-2
RW10434_RNAi_29	pdi-3, pdp-1, pfd-3, pfd-6, pfkb-1.1, pgk-1, pgp-2, pgrn-1, pgs-1, phip-1, pif-1, pigv-1, plk-2, pmr-1, png-1
RW10434_RNAi_30	pnk-1, pole-1, pop-1, ppfr-1, ppfr-2, pqn-44 (tent-5), prg-1, pri-2, prmt-7, prp-17, prp-31, prp-4, prpf-4, psf-2, ptps-1
RW10434_RNAi_31	ptr-2, ptr-21, ptr-23, R05D11.4 (ddx-52), R06C7.6, R119.2, R119.5, R12E2.11, R12E2.13, rab-10, rab-11.1, rab-5, rab-8, rad-54 (rad-54.L), rad-8
RW10434_RNAi_32	rae-1, ran-4, rba-1, rbg-2, rbpl-1, rbx-2, rcor-1, rga-2, rig-5, rla-0, rnp-6, row-1, rpb-10, rpb-5, rpb-7
RW10434_RNAi_33	rpl-25.2, rpl-27, rpl-30, rpl-31, rpl-4, rpn-10, rpn-8, rpoa-2, rpom-1, rps-17, rps-6, rskn-1, rsr-1, rtcb-1, rtel-1
RW10434_RNAi_34	sac-1, sacy-1, scpl-1, scpl-3, sdha-2, sec-12, sec-8, sem-2, sesn-1, set-10, set-18, sin-3, sir-2.4, skr-1, skr-2
RW10434_RNAi_35	slx-1, smd-1, smg-1, smg-2, smgl-1, smn-1, smo-1, smp-1, smu-1, snf-1, snpc-4, snrp-27, snx-27, snx-3, sod-2
RW10434_RNAi_36	spe-5, spg-7, sqv-5, stam-1, stl-1, stn-1, suco-1, sup-17, sur-2, sur-6, swsn-9, syx-7, T01G9.2, T01H8.2 (serr-1), T03F1.12
RW10434_RNAi_37	T05E7.3, T08B2.5 (rbm-5), T08G11.1, T08G11.4 (tgs-1), T09B4.8, T10B11.2 (cerk-1), T10B11.6, T19B4.3, T22C1.1, T22C1.6, T23G11.4, T23H2.3, T24B1.1, T25G3.3, T25G3.4 (gpdh-3)
RW10434_RNAi_38	T26E3.4, T27A3.6 (nmrk-1), T27F6.6, tads-1, taf-1, taf-11.2, taf-4, tag-173 (bckd-1B), tag-179 (algn-10), tag-296, tat-5, tbca-1, tbcd-1, tct-1, tdpt-1
RW10434_RNAi_39	teg-4, thoc-3, tin-13, tin-44, tlf-1, toe-4, tol-1, top-1, tppp-1, trak-1, tre-1, trpp-10, trpp-8, ttm-5, ttx-7
RW10434_RNAi_40	tub-2, txl-1, tyms-1, uba-2, uba-5, ubc-12, ubc-16, ubc-25, ubc-3, ubl-5, ubql-1, ubr-1, C44E4.1 (ubr-4), ubxn-1, uev-1
RW10434_RNAi_41	unc-101, unc-108, unc-11, unc-37, unc-40, unc-54, unc-59, unc-73, unc-75, unc-94, usp-3, usp-5, vha-10, viln-1, vpr-1
RW10434_RNAi_42	vps-20, vps-25, vps-28, vps-34, vps-4, vps-51, W02A11.1, W02D3.4, W02D9.2, W04A4.5 (ints-4), W08E3.2 (casc-3), W09C5.7 (sac-2), W09C5.8 (cox-4), W09G3.6, W09G3.7
RW10434_RNAi_43	wdr-12, wdr-23, wts-1, wve-1, wwp-1, xpa-1, xpo-2, Y105E8A.11, Y105E8A.14, Y105E8A.2, Y105E8A.20, Y105E8A.25, Y105E8A.3 (slc-30A5), Y105E8A.8 (tcab-1), Y105E8B.7
RW10434_RNAi_44	Y106G6D.5, Y106G6D.7 (rbm-12), Y106G6E.4, Y106G6H.14, Y106G6H.5 (pdpr-1), Y106G6H.8, Y110A7A.9, Y119C1B.5 (rnf-145), Y18D10A.3, Y18D10A.9 (ciao-1), Y23H5B.5, Y23H5B.6 (ddx-10), Y34D9A.7, Y37E3.1, Y40B1B.8 (slc-25A46)
RW10434_RNAi_45	Y44E3A.4 (cdap-2), Y44E3A.6 (edc-4), Y47D9A.1, Y47G6A.18, Y47G6A.7, Y47H9C.7 (eif-2Bbeta), Y48G10A.1, Y48G10A.3, Y48G1A.4 (nol-14), Y48G1C.10, Y48G1C.12, Y48G8AL.13, Y48G8AL.5 (nsun-2), Y52B11A.10, Y52B11C.1 (pigl-1)
RW10434_RNAi_46	Y54E10A.6, Y54E10BR.2, Y54E10BR.3, Y54E10BR.4, Y54E5A.5, Y54E5A.6 (dus-2), Y54E5A.7 (gid-1), Y63D3A.7, Y65B4A.1 (cus-2), Y65B4A.6, Y65B4A.8, Y6B3B.4, Y6B3B.5 (gpap-1), Y6B3B.9, Y71F9AL.1
RW10434_RNAi_47	Y71F9B.2, Y71F9B.6, Y73E7A.2 (aatf-1), Y87G2A.13, Y87G2A.2, Y92H12A.2, Y92H12A.4 (ints-3), Y95B8A.8 (zfr-1), yop-1, ZC434.8, zfh-2, zhp-3, ZK265.6 (nol-16), ZK524.4, ZK858.6
RW10434_RNAi_48	ZK858.7, ZK909.3, ZK973.1, ZK973.11, ztf-11
RW10434_RNAi_add	ctr(RNAi), C30H7.2 (erp-44.1), C44E4.5, F15D3.6, gst-43, lpd-3, M04F3.4, mafr-1, ndx-7, pash-1, ptr-23, rig-5, tag-173 (bckd-1B), vpr-1, Y34D9A.7, Y48G1A.4 (nol-14)