F19F10.9

WormBase ID: WBGene00017605

WormBase description: Is an ortholog of human SART1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP). Is predicted to encode a protein with the following domain: SNU66/SART1 family.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AACACTTACCTTTTCGATCG

sgRNA2: GATGATGACACTTCTGAAGT

5'HR: GCATGCTGAAATGGAGGATTTGGAAATTTTCTGAAAAACGGAAAAAGGTTGAAAATTATGAAACATAATTTTTATTTTATAAACTGACCCACAAAACTCTTCTCTTTTCCATTTTTTATCACTTGGCAATACTTCAATTCGAGAAATGAACTCGAGCATTTCCTCCATCAAAAGTCTGTAAATTTTTATCTAGCAGTTGTAATATCCAATTTCTTATATCACTCACTTTCCGTACTGATACGCAATCTCCGTAACATAAATTTTCCCAGAAAATCCGGAACCCTCTGTGTAGAACGGCAAACCGACGAATGATTCATAGTTGGACACCAAAATTGCGTCGATTGTATCCATTTTTAGCATATCCGTCTGAAATGCGTCTAGTATTAAATTTAATTAATCTCCATGTTTACAAACCGATACGTGGAAAATTTCAGGTGGCGATTCGACGTAGACACGGTTTCCGAGCTCTTTCAGATACGGTATCTGAAACTTCTTGGCGATCGGAGCATTTTTGATTCGCGGGCTGAAATTTTTTGCTTTGAAAAGAAACGACGTCACTTTTCCTTGATTTACCTTTGATATACATGTGGGAGGAAGCTGAAAAATGGTGTGAAATCAATCGGAGTGTCCATAAGAATTCTGGCATTTGGCCATTCTAGCAGAAAACATGGTTTTTGTGCGTACACCGAGAAGTTTGTCTGAAATTAGTTATTTATTCCATCTGTCAAGTAGTTTTTTAATCAAAAACCTACGATATTCATTATTTTAGTCTTGTTTCAGCGCCCTTCCATTAATTTTTAGATAAATACAAAAAATAAGTTCGAATTAAATTGGATTCTGCGCTAAGTGCGGGTGCTGACGCATTACGCGATACTGTTTGCGTTATTTTCGAGATTTATTTTCGAAAGTGTCCCATTCAAGTTTCAGTCAGTTTCAGCCAAGTTTTCATTGATTTTTCTCATTTTAATCTTGAAAGGTACC

3'HR: CTGCAGATGTCTTCCAAATACTCAAAAAAACGAAAACAGTTCGATGATGATACCTCCGAGGTTGGAAAAAATCGAGATTCATCAAGATCTCCATCTCCTCCACGCTCAAAGAGGTAAGTCTTTTTGTTACTTTTTCTTTTTATTAATTCTGTAATTCACAGGTGAAATTGCAGATCTGCGAAACAGCGAGAGCCTGAGGATAATGGAGAACCGTAAGTATAACACGGAGAGCATTTCATTCAATAAAATAATTATTTTTCAGGGCGGATTCGATGTCAATCGAAGAAACAAACAAACTGCGCGCCTCGCTTGGTTTGGCTCCTCTTGAAACTAACGACAATAAGGAACGTGATGCTAATGATGGAACCAATGAGAAAATCTATAATGAGGATGGATTTGAGTTCAGACATCGAAAAGCTGAGAATATTTCTGATAAGAAAAAAGAAAAAGATATCAAAGAGAAGCTAGAAATCGCCAAAGCGAAACGAGATGTTTATTCAAAAGTCCTGAAAGCGAAGAAACTCGCAGATTCTGATGAAGAAGACAGTGCAGCCAGTTTTGTGAACAAAATGCGTCAGAAAGAAGATGAAGCCAAAAAGGCCGCCGATAGAGCCGCAGCTTTTGATCAACTTGACGAAGAGCTTGGTGTCAGTGCAATCGTCGATAAAGTGAAAAAGCCGAAGAAACCAAAAACCAAAGGGCCATCTGATACAGCTGGAATGGTTATTGGTCATGGGAGAGAGGCTTTTATTGAAGGTGATCAAATTTTGGTGCTTCAAGATAAGGGAGTTCTTGATGATGGAGACGAAGTTCTCGTGAATCCAAATCTGCTAGACAATGAACGTCACGTCAGAAATGTTGAATTGAGGAAACGGAAGGATCCTAATCGGAATTTTGATGAAGATGTCGACGAGTTTGGAAATGTAGGGATTTTATTTTAAAAGTTTAACTGATTACTAGT

KI verification primers: F19F10.9-NKIF: 5'-GTGCATATCAGTTGTCGTGTAGTAGG-3' F19F10.9-NKIR: 5'-CCAACGTCTCATCGTACTTTGC-3'

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Strain name: SYS423

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; F19F10.9 (devKi94 [mNeonGreen::F19F10.9]) V

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: PHA-4

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: PHA-4, DSC-1, ELT-3, FOS-1, LIN-13, NHR-85, ZTF-11