KI information: mNeonGreen knockin at N-terminus
sgRNA1: AACACTTACCTTTTCGATCG
sgRNA2: GATGATGACACTTCTGAAGT
5'HR: GCATGCTGAAATGGAGGATTTGGAAATTTTCTGAAAAACGGAAAAAGGTTGAAAATTATGAAACATAATTTTTATTTTATAAACTGACCCACAAAACTCTTCTCTTTTCCATTTTTTATCACTTGGCAATACTTCAATTCGAGAAATGAACTCGAGCATTTCCTCCATCAAAAGTCTGTAAATTTTTATCTAGCAGTTGTAATATCCAATTTCTTATATCACTCACTTTCCGTACTGATACGCAATCTCCGTAACATAAATTTTCCCAGAAAATCCGGAACCCTCTGTGTAGAACGGCAAACCGACGAATGATTCATAGTTGGACACCAAAATTGCGTCGATTGTATCCATTTTTAGCATATCCGTCTGAAATGCGTCTAGTATTAAATTTAATTAATCTCCATGTTTACAAACCGATACGTGGAAAATTTCAGGTGGCGATTCGACGTAGACACGGTTTCCGAGCTCTTTCAGATACGGTATCTGAAACTTCTTGGCGATCGGAGCATTTTTGATTCGCGGGCTGAAATTTTTTGCTTTGAAAAGAAACGACGTCACTTTTCCTTGATTTACCTTTGATATACATGTGGGAGGAAGCTGAAAAATGGTGTGAAATCAATCGGAGTGTCCATAAGAATTCTGGCATTTGGCCATTCTAGCAGAAAACATGGTTTTTGTGCGTACACCGAGAAGTTTGTCTGAAATTAGTTATTTATTCCATCTGTCAAGTAGTTTTTTAATCAAAAACCTACGATATTCATTATTTTAGTCTTGTTTCAGCGCCCTTCCATTAATTTTTAGATAAATACAAAAAATAAGTTCGAATTAAATTGGATTCTGCGCTAAGTGCGGGTGCTGACGCATTACGCGATACTGTTTGCGTTATTTTCGAGATTTATTTTCGAAAGTGTCCCATTCAAGTTTCAGTCAGTTTCAGCCAAGTTTTCATTGATTTTTCTCATTTTAATCTTGAAAGGTACC
3'HR: CTGCAGATGTCTTCCAAATACTCAAAAAAACGAAAACAGTTCGATGATGATACCTCCGAGGTTGGAAAAAATCGAGATTCATCAAGATCTCCATCTCCTCCACGCTCAAAGAGGTAAGTCTTTTTGTTACTTTTTCTTTTTATTAATTCTGTAATTCACAGGTGAAATTGCAGATCTGCGAAACAGCGAGAGCCTGAGGATAATGGAGAACCGTAAGTATAACACGGAGAGCATTTCATTCAATAAAATAATTATTTTTCAGGGCGGATTCGATGTCAATCGAAGAAACAAACAAACTGCGCGCCTCGCTTGGTTTGGCTCCTCTTGAAACTAACGACAATAAGGAACGTGATGCTAATGATGGAACCAATGAGAAAATCTATAATGAGGATGGATTTGAGTTCAGACATCGAAAAGCTGAGAATATTTCTGATAAGAAAAAAGAAAAAGATATCAAAGAGAAGCTAGAAATCGCCAAAGCGAAACGAGATGTTTATTCAAAAGTCCTGAAAGCGAAGAAACTCGCAGATTCTGATGAAGAAGACAGTGCAGCCAGTTTTGTGAACAAAATGCGTCAGAAAGAAGATGAAGCCAAAAAGGCCGCCGATAGAGCCGCAGCTTTTGATCAACTTGACGAAGAGCTTGGTGTCAGTGCAATCGTCGATAAAGTGAAAAAGCCGAAGAAACCAAAAACCAAAGGGCCATCTGATACAGCTGGAATGGTTATTGGTCATGGGAGAGAGGCTTTTATTGAAGGTGATCAAATTTTGGTGCTTCAAGATAAGGGAGTTCTTGATGATGGAGACGAAGTTCTCGTGAATCCAAATCTGCTAGACAATGAACGTCACGTCAGAAATGTTGAATTGAGGAAACGGAAGGATCCTAATCGGAATTTTGATGAAGATGTCGACGAGTTTGGAAATGTAGGGATTTTATTTTAAAAGTTTAACTGATTACTAGT
KI verification primers: F19F10.9-NKIF: 5'-GTGCATATCAGTTGTCGTGTAGTAGG-3' F19F10.9-NKIR: 5'-CCAACGTCTCATCGTACTTTGC-3' Download
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