WormBase ID: WBGene00004804
WormBase description: Exhibits Hsp70 protein binding activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including endoplasmic reticulum unfolded protein response; positive regulation of cellular response to manganese ion; and positive regulation of transcription from RNA polymerase II promoter in response to heat stress. Localizes to endoplasmic reticulum; mitochondrion; and nucleus. Is expressed in several structures, including germ line; head ganglion; intestine; neurons; and pharynx.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: GAGGGCTTCTCAGATGTAAT
sgRNA2: CACTTGCCGTCAAAGACGCA
5'HR: GCATGCAAAATTTCCAGTGTTCCCAACATCCAACTACGCCTACATTGGAATGCAAAACGACAGTCTTCAAGCAGTTGTATCAAATGGACAGATTGACTATGATCATTCCTATCAATCCACTGGTCAGACTCCACTGTCTCCTCTCATCATTGGATCTTCAGGACGTCAACAGCAGACTCAAACGAGCCCAGGAAGCGTCACAGTGACTGCAACAGCTACTCAATCGTTGTTCGATCCATATCACTCACAGAGACACTCGTTTAGTGATTGCACTACGTAAGTTGCTCAGTCTGAAAGTCTAGAAAGTCTCTTGGGATCCCATTCAGCTTATTAGTAAAAGTGCACTTGCTCAAATAGGGCAAGTGGGTACCCATGGGAACAGTTTTCAAAGTACTTTTATGCAAAAGTTCAATAATTACATAATTTCAGTGATTCGTCATCAACGTGCTCTCGCCTCTCTTCGGAATCTCCACGATACACGTCAGAGAGCTCAACCGGAACTCACGAGTCTCGTTTCTACGGAAAGTTGGCTCCATCCAGTGGATCACGCTACCAACGATCATCGTCTCCACGTTCATCACAATCTTCGATTAAGATCGCGAGAGTTGTTCCACTGGCCAGCGGACAACGGAAGCGTGGACGTCAATCCAAGGATGAGCAGCTCGCCAGTGACAACGAGCTTCCAGTGTCGGCGTTCCAGATTTCGGAGATGTCATTAAGCGAGTTGCAACAAGTGTTGAAGAACGAGAGTCTCAGCGAGTATCAAAGACAGTTGATTCGCAAGATTCGTCGACGCGGTAAGCATCATTATTGATGACAGGATCGAAATGATTTTTTTTGTAATTTCAGGAAAGAACAAGGTTGCTGCCCGCACTTGCCGTCAAAGACGCACAGATCGTCACGACAAGATGTCCCATTACATCACC GGT
3'HR: CTGCAGGAAGCCCTCTTTTATCACATAAAATCTCGGTCGAAACCTTATTAAAGCCACATAATTAAAGATAATTAATTCCGCCACAATAATCGTTTTTTTCTTCTTTGCCGTGTCTCATTTCATTTTGATCTACTCTTTCCTCCCTTCGGATTCTTTGATTTCCCAGTGAAATACCTCACCCACTTCAATCCCCACAAAGTGAGCAACCCCTATCTTGCAACAGTTTTATCATCTCTTCATCATACCCAGTTTGATAATTTATTATCTGATCCCCATCCCCTTGTCGCCTCTCATTAGTATCCTAGTTTTTCATTTGAGCCCGGAGCTCAGACTACATCTCCGAATCATCATACAAATAGATAGAAACGGGTCTCGTGACGAAAGAATACGTGCACCACACGACCCCCCCATCCTGTTCACCCCCATACACCTGAAAAATATGATCTTTACAGTTATTTCTATTATATCCTCAAATCTCTCGTAATATCGTATCAATTTCCTCTTCTTTTTTGTCATTTTCAATTTTTCTCAAATTTCTCAGATCTATTCTTTTTCTTGTATTTTTGGAACTTGTATCCCTCCTCCATCCCCAGACTTCCCCTTCCCAGTTACTCTTGTACATTTTCATATATGTCCATATATCGTTTGAATCTCTCATTTATGGAAATAAATTTGAAAAAATCAATCATTGAAAAGCAAAAATTTCATTCAAATAAAATGATGTTGGTGAATTTAACACAGAAAACAGGGGGACTTGATTTATAAATATAGACAAAACGAGAATTAAGCATCGGCCATCTCAACAGCGGGCTCCGGAGCAGATGGAGAAGGACCAGCCTTCTCGGGTTCAGTTGCCACGGCGATATCCATTGGAGATCACTAGT
KI verification primers: SKN-1-CKIF: 5'-AGACATTCCAACATTTCCTCCTT-3' SKN-1-CKIR: 5'-GATGCAAAGGCAGTCGGAGAT-3' Download
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Strain name: SYS515 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; skn-1 (devKi152 [skn-1::mNeonGreen]) IV
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Expression pattern: Expressed after bean stage
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-44, Y73E7A.1, DPFF-1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: BCL-11, BLMP-1, CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, COG-1, DVE-1, ELT-1, ELT-2, ELT-3, HAM-2, HLH-30, NFYA-1, PHA-4, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: BCL-11, BLMP-1, CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, COG-1, DVE-1, ELT-1, ELT-2, ELT-3, HAM-2, HLH-30, NFYA-1, PHA-4, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62
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