UNC-30

WormBase ID: WBGene00006766

WormBase description: Is an ortholog of human PITX1 (paired like homeodomain 1). Exhibits sequence-specific DNA binding activity. Is involved in neuron differentiation; positive regulation of transcription by RNA polymerase II; and regulation of locomotion. Localizes to nucleus. Is expressed in intestine and neurons. Human ortholog(s) of this gene are implicated in clubfoot.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TGCCACAGCTTTCTTCAACG-3'

Strain name: SYS69

Cross: OP395 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs395 (unc-30::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LET-381, HND-1, TBX-7, ALR-1, UNC-39, M03D4.4, UNC-3, CND-1, VAB-3, COG-1, CEH-36, UNC-42, CEH-13, HLH-3, NGN-1, CEH-48, TLP-1, HLH-2, ZTF-11, EFL-3


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ALR-1, ATHP-1, CEH-38, RCOR-1, Y55F3AM.14

Targeted by: CND-1, CEH-36, UNC-42, LSY-2, NFYA-1, UNC-62, ZIP-8, BLMP-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ATHP-1, RCOR-1

Targeted by: CND-1, CEH-36, UNC-42, LSY-2, NFYA-1