ZIP-8

WormBase ID: WBGene00017755

WormBase description: Is predicted to have DNA-binding transcription factor activity. Is expressed in intestinal cell.

Reporter verification: Whole-genome sequencing indicated that the GFP tags another TF, ZIP-8 rather than CEBP-2
Primers used to verify fosmid reporter: 5'-AAAGCGTATGAAGAATAATGAGGC-3'

Strain name: SYS454

Cross: OP329 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs329 (zip-8::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-8, VAB-3, ALR-1, CEH-31, CEH-10, PAG-3, MBF-1, UNC-42, Y73E7A.1, CEH-41, HLH-30, SEM-2, TTX-3, Y56A3A.28, LIN-32


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-8, VAB-3, PAG-3, UNC-42, HLH-30, SEM-2, LIN-32, AHR-1, ATF-7, ATFS-1, BCL-11, BED-3, CAMT-1, CEH-16, CEH-19, CEH-27, CEH-30, CEH-32, CEH-34, CEH-36, CEH-40, CEH-43, CEH-45, CEH-53, CEH-6, CEH-9, CES-2, CND-1, COG-1, DAF-12, DMD-4, DMD-5, DUXL-1, DVE-1, EFL-3, EGL-13, EGL-38, EGL-44, EGL-5, ELT-3, END-1, EYG-1, FKH-10, FKH-2, GMEB-2, HAM-2, HLH-14, HLH-2, HMBX-1, LAG-1, LET-381, LIM-7, LIN-11, LIN-13, LIN-31, LIN-48, LSL-1, M03D4.4, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NGN-1, NHR-102, NHR-25, NHR-49, NHR-67, NHR-76, NHR-85, NOB-1, PAL-1, PES-1, PHA-4, POP-1, REF-2, SEL-8, SOX-4, SPTF-1, SYD-9, TBX-11, TBX-37, TBX-7, TTX-1, UNC-130, UNC-30, UNC-62, UNC-86, ZAG-1, ZTF-11, ZTF-30, ZTF-6

Targeted by: CEH-36, CND-1, ELT-1, REF-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-8, VAB-3, UNC-42, LIN-32, AHR-1, ATF-7, ATFS-1, BCL-11, BED-3, CAMT-1, CEH-16, CEH-19, CEH-27, CEH-30, CEH-33, CEH-34, CEH-40, CEH-5, CEH-9, CES-2, CND-1, DMD-5, DUXL-1, DVE-1, EFL-3, EGL-13, EGL-44, EGL-5, ELT-3, END-1, EYG-1, HMBX-1, LET-381, LIN-31, LIN-48, LSL-1, MDL-1, MEF-2, MML-1, NHR-25, NHR-49, NHR-85, NOB-1, PAL-1, PES-1, POP-1, REF-2, SOX-4, SYD-9, TBX-11, TBX-37, TBX-7, UNC-62, UNC-86, ZTF-30

Targeted by: CND-1, ELT-1